Optimizing tissue culture protocol for in Vitro shoots and roots development of sugarcane (Saccharum officinarum L.)
DOI:
https://doi.org/10.63147/krjs.v4i3.93Abstract
Sugarcane (Saccharum officinarum) contributes 60–70% of annual sugar production in the world. In the present study, a complete optimization protocol of sugarcane cultivar HSF-240 was designed for in-vitro micro-propagation under different media concentrations. Young leaves were sterilized by using various concentrations of Clorox. The results revealed that almost all contamination was controlled by using antibiotic cerfotaxime (500mg-1) in the initiation step of the culture medium. However, for callus induction, variable concentrations of 2,4-D were used. It was observed that a lower concentration of 3mgl-1 2,4-D, showed better results than a higher concentration. The shoot proliferation and multiplication were obtained by using various hormonal blends. The results revealed that RM12 (BAP 1mgl-1 +KIN 0.5mgl-1) exhibited good result. The highest shoot length and shoot number were obtained by BAP and KIN combination as compared to individual BAP and KIN. Similarly, root induction obtained by eight different hormonal applications of NAA and IBA. It was shown that the highest root length and root number were obtained at IBA (0.5mgl-1) and by NAA (2mgl-1). In conclusion, the current study revealed that diverse hormonal concentrations significantly affect the in-vitro growth of explants of sugarcane. It is suggested that further comparative investigations are required to understand the nutrient contents of sugarcane in a controlled environment and in soil for better acclimatization and the highest yield in a natural habitat.
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